Overexpression of MtHSFA9 in hairy roots of Medicago truncatula

HSFA9 (Medtr4g126070) is a seed-specific heat shock transcription factor that is upregulated during the later stages of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Medicago hairy roots were generated using Agrobacterium rhizogenes transformed with the genomic sequence of the genomic HSFA9 gene of Medicago. Using the Medicago NimbleGen chip, a transciptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control Two-condition experiment, gus versus genomic. Biological replicates: 3 control (35s :: GUS-GFP), 3 treatment (35s :: Genomic-GFP), independently grown and harvested. One replicate per array.