five

S3_File: ground truth manual segmentation data on 2D MIP images

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DataONE2016-06-20 更新2024-06-26 收录
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This is a zip-file containing manually segmented 2D ground truth labeled masks of 3D spheroid cultures. The cultures are LNCaP cancer spheroids in co-culture with CAF stromal cells (untreated, DMSO samples). For the manual segmentation 2D maximum intensity projections (MIPs) were used of the original 3D stacks. The spheroids are labeled in five distinct classes: (1) well separated, (2) overlapping with brighter spheroids in the MIP (rendering it non-separable), (3) overlapping with less bright spheroids in the MIP (rendering it well separable), (4) merely touching other spheroids, and (5) touching the border of the 2D projection of the image. Opening of the images in FIJI (ImageJ) with the ROI Manager allows visual inspection of the data. For this purpose the cases (1) to (5) are color-coded in red, green, magenta, cyan, and blue, respectively.
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2016-06-20
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