Global promoter usage in the differentfractions of the cell during early zebrafish development

We employed transcriptomics methods to examine differences in promoter usage in the nuclear and cytosolic fractions of zebrafish embryonic cells at different stages of development. The analysis of the CAGE-seq data revealed differences in promoter width within the same stage (Post-MZT) in different compartments of the embryonic cell proposing a spatial and temporal regulation of gene expression. Overall design: Promoter organisation in the nuclear and cytosolic fractions of zebrafish embryonic cells in the 64 Cell, 256 Cell, 1000 Cell, Dome, Shield and Prim-5 stages of development.