SHH is downstream of immediate early genes and is targeted by chemotherapy on a specific enhancer [CUT&Tag, ATAC-seq]

Sonic Hedgehog (SHH) signaling is a key regulator of embryonic development and tissue homeostasis, and is involved in gastrointestinal (GI) cancer progression. Here we show that SHH gene expression is down-regulated by chemotherapy drugs. The 139Kb upstream enhancer is specifically targeted by the immediate early gene EGR1. Human CRC cell line HT29 was cultured and treated with 5FU for 2h or 4h, and subjected to ATAC-seq or Cut-tag analysis using the corresponding antibodies.