CRSP: Comparative RNA-seq pipeline for species lacking both of sequenced genomes and reference transcripts. CRSP: Comparative RNA-seq pipeline for species lacking both of sequenced genomes and reference transcripts

RNA sequencing (RNA-seq) has been a widely used high-throughput method to characterize transcriptomic dynamics spatiotemporally. However, typical RNA-seq data analysis pipelines depend on either a sequenced genome or reference transcripts. This constriction makes the use of RNA-seq for species lacking both of sequenced genomes and reference transcripts challenging. To solve this problem, we developed CRSP, an RNA-seq pipeline integrating multiple comparative species strategy but not depending on a specific sequenced genome or reference transcripts. Benchmarking suggests the CRSP tool can achieve high accuracy to quantify gene expression levels. Overall design: Human ES (H1) RNA-seq