MNIC yields (pmol/108 cells) in rat BM suspensions and EC cultures.
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*Technical footnote: Since proper eNOS activity requires EC attachment and cell-cell contact, NO trapping was done on monolayers of HUVEC and bEnd.3 cells firmly attached to the bottom of a culture T75 flasks, as described previously [21]. A single flask with ca 7.5×106 confluent bEnd.3 cells has adequate signal to noise ratio [21], whereas HUVEC, having less eNOS, requires at least two flasks with total 15×106 cells.
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2015-12-02
