Chromatin architecture landscape in hierarchical hematopoietic cells

The spatiotemporal chromatin reorganization during hematopoietic differentiation has not been well characterized, which partly limited by the large amounts of starting cells for the current high-throughput chromatin conformation capture approaches. Here, we introduce a low-input Hi-C method, tagHi-C, to capture the chromatin structures in hundreds of cells. We proved tagHi-C was a feasible and reproducible approach. Overall design: We applied tagHi-C in 10 mouse adult hematopoietic cell populations at different stages to comprehensively map the 3D chromatin landscape during normal hematopoiesis.