Effects of AEBP1 knockdown on gene expression profiles in HUVEC

We identified adipocyte enhancer binding protein 1 (AEBP1) as a novel tumor endothelial marker in colorectal cancer (CRC). To identify target genes of AEBP1 in endothelial cells, we knocked down AEBP1 in human umbilical vein endothelial cells (HUVECs). We found that genes associated with angiogenesis including aquaporin 1 (AQP1) and periostin (POSTN) are downregulated by AEBP1 knockdown in HUVECs, suggesting that AEBP1 may promote tumor angiogenesis through regulating these genes. HUVECs were transfected with a control siRNA (Silencer Select Negative Control No. 1 siRNA, Thermo Fisher Scientific) or siRNAs targeting AEBP1 (Silencer Select Pre-designed siRNA; AEBP1 siRNA1, s1145; AEBP1 siRNA2, s1146; Thermo Fisher Scientific) using HUVEC Nucleofector Kit (Lonza) and incubated for 48h or 72h.