Single-cell RNA-Seq profiling to characterize mammary tumor infiltrated immune cells with iron supplement during anti-PD-1 immunotherapy

In previous study, we demonstrated that iron supplementation augmented the antitumor effect of PD-1 based immunotherapy in triple-negative breast cancer models. To gain a more profound understanding into whether and how iron plus PD-1 antibody combination influences tumor immune microenvironment to exert better therapeutic activity, in this study, we performed single cell RNA-sequencing with PIP-seq to profile and compare tumor infiltrated CD45+ cells derived from control, iron or PD-1 antibody alone, and combination treated E0771 tumors. Overall design: Tumor infiltrated CD45+ cells derived from control, iron or PD-1 antibody alone, and combination treated E0771 tumors were flow sorted and analyzed by single cell RNA-sequencing using PIP-seq pipelines. Cells from three or four tumors of each treatment group were pooled for PIP-seq.