High-throughput lncRNA sequencing profile of ovarian granulosa cells in polycystic ovary syndrome

Purpose:To examine different expression profiles of lncRNAs and mRNAs in ovarian granulosa cells (GCs) in PCOS patients and controls Patients:6 GCs samples in each group were subjected to high-thoughput sequencing Results:54,615 lncRNAs and 123,141 mRNAs were detected in this high-thoughput sequencing, and we identified 1049 differential expressed lncRNAs and 3246 mRNAs (fold-change?2, P-value<0.05). So far, we found 7 lncRNAs and 3mRNAs were validated consistent with sequence profile which were selected, randomly. There is still a large part that we have not verified yet. Conclusion: Our study provides the detail analysis of GCs transcriptomes, generated by high-thoughput technology. This report also provides a framework for comparative investigations of PCOS. Our results revealed the expression of lncRNAs, mRNAs and part of circRNAs in PCOS, after more in-depth research in future, it can provide basic datas for studying the pathogenesis of PCOS. Overall design: The expression profile of mRNAs, lncRNAs in GCs of PCOS patients