P4PP: an universal shotgun proteomics data analysis pipeline for virus identification, samples of DGA CBRN Defence Center

Humans can be infected by a wide variety of virus species. To detect these different viruses, a data analysis approach for shotgun proteomic data was developed. For this proteome for pandemic preparedness (P4PP) pipeline, a database (P4PPv01) and a web application (P4PP) were constructed. The P4PP pipeline enables the identification of 1896 virus species from the 32 virus families in which at least one human-infectious virus is described. To evaluate the developed analysis pipeline the supernatant of Vero Cell cultures of three Togaviridae (Rio Negro virus, Sindbis virus and Pixuna virus) and a negative sample were analyzed.