Summary of nested PCR analysis and sequencing for HPV16/18, and Beta-PV types.

-, sample is negative for this PV-type. AKT1 indicates the presence (pos) or absence (neg) of AKT1 expression in the epidermis of the VIN/vSCC. Copy no. indicates the HPV16 copy number per cell in each sample. Integrated? denotes the integration status of HPV16 based on the HPV16 genomic PCR. N.B. Several samples were PCR-positive for cutaneous alpha-PV species 4, however sequencing showed this was due to non-specific amplification of HPV16 in all cases; gamma and mu/nu types were not detected. E1-E4 and E7, summary of the immunohistochemistry for HPV16 EE4 and E7. n.d – not determined.