Single cell genome sequencing of murine lymphoma and epidermal cells to identify copy number variations in individual cells. Single cell genome sequencing of murine lymphoma and epidermal cells

Centrosome amplification is a common feature of human tumors, but whether this is a cause or a consequence of human cancer remains unclear. Here, we report the creation of a mouse model in which centrosome number can be persistently increased in the absence of additional genetic defects. We show that extra centrosomes increase tumor initiation in a mouse model of intestinal neoplasia. Mice overexpressing a doxycycline-inducible Plk4 transgene were monitored for the development of cancer and other pathologies. Tissues of five mice were examined: three untransformed epidermal samples, one B-cell lymphoma and one T-cell lymphoma. All tissues were harvested, homogenized and single-cell sequenced as described before (see Bakker & Taudt et al., 2016 – Genome Biology 17:115).