RNA-seq analysis using A549 cells with FXR knockdown and control A549 cells with or without ionizing radiation treatment.

In this study, we explored the role of FXR in the response to ionizing radiation (IR) in A549 human lung cancer cells. FXR was stably knocked down in A549 cells using shRNA, and four experimental groups were established: control A549 cells (non-irradiated), control A549 cells (irradiated), FXR knockdown A549 cells (non-irradiated), and FXR knockdown A549 cells (irradiated). RNA sequencing (RNA-seq) was performed to identify gene expression changes associated with FXR knockdown and irradiation. This dataset provides insights into the molecular mechanisms by which FXR influences the cellular response to radiation and its potential impact on cancer therapy. Overall design: The experiment was designed to investigate the impact of FXR knockdown on the gene expression profile of A549 cells following ionizing radiation. A549 cells were stably transfected with shRNA targeting FXR or a control shRNA. The experiment included four groups: (1) control A549 cells without irradiation, (2) control A549 cells exposed to irradiation, (3) FXR knockdown A549 cells without irradiation, and (4) FXR knockdown A549 cells exposed to irradiation.Each group included three biological replicates, totaling 12 samples. RNA was extracted from all 12 samples and subjected to RNA sequencing to compare the transcriptomic changes induced by FXR knockdown and ionizing radiation. This design allows for the analysis of the specific role of FXR in the cellular response to radiation.