Fig 3: Stability of mEPSC amplitude after prolonged periods of sleep or wake

The left column represents example sleep histories for the VC L2/3 experiments while right column shows mEPSC characteristics for the indicated cell types and brain regions. (A) Top figure, hypnograms for three animals used for sleep dense mEPSC recordings. Green bars indicate instances of wake, magenta indicates REM, and blue NREM. Bottom, this same behavioral state data represented as a moving average of % time spent awake in the preceding 4 hr; the dashed lines represent the thresholds for achieving the required density in a given state, and the dots represent endpoints for slice recordings. (B) and (C) are same as for (A), but for wake dense recordings performed during the light and dark periods, respectively. Red arrow indicates wake encouragement (introduction of toy). Black circle around end point dot indicates which trace is represented by example hypnogram. (D) Traces on the left, example mEPSC recordings from L2/3 of V1; scale bar, 10 pA, 1 s. On the right, cell average mEPSC events; scale bar, 5 pA, 5 ms. (E) On left, cell average mEPSC amplitudes from L2/3 V1; error bars = 95% CI of mean (p=0.17; Kruskal-Wallis). (E) On right, cumulative histogram of sampled mEPSC amplitudes (SD – L. WD p=0.075, L. WD – D. WD p>0.9, SD – D. WD p=0.77; KS test with Bonferroni correction). Inset for (E) – (G) is a peak scaled average mEPSC waveform; scale bar, 50% peak, 5 ms. Sleep dense n = 17 cells, 3 animals; L. wake dense n = 25 cells, 4 animals; D. wake dense n = 18 cells, 5 animals. (F) On left, cell average mEPSC from L4 V1 (p=0.52; Kruskal-Wallis). (F) On right, cumulative mEPSC amp. from L4 V1 (p=0.99; KS test). Sleep dense n = 47 cells, 6 animals; L. wake dense n = 32 cells, 4 animals. (G) On left, cell average mEPSC amplitudes from L2/3 PFC (p=0.13; Kruskal-Wallis). (G) On right, cumulative histogram of sampled mEPSC amplitudes (SD – L. WD p>0.9, L. WD – D. WD p=0.096, SD – D. WD p=0.015; KS test with Bonferroni correction). Sleep dense n = 31 cells, 6 animals; L. wake dense n = 28 cells, 5 animals; D. wake dense n = 18 cells, 6 animals.