Expression data of mRNA from twins with tuberculosis infection or not

To search the genes which were associated with tuberculosis infecion We used the microarrays to detail the global programme of gene exprssion between with the tuberculosis infecion and without the tuberculosis in monozygous twins Total blood RNA was extracted using the PAXgene RNA Blood Mini Kit (Qiagen, Venlo, The Netherlands), according to the manufacturer’s instructions. The amount of RNA was quantified by the spectrophotometric A260 to A280 ratio of 1.8, whereas quality was assessed using denaturing gel electrophoresis using the Agilent 2100 system (Agilent Technologies, Santa Clara, CA, USA). Gene expression profiles were analyzed by PrimeView Human Gene Expression Array (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.