Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast

Fluorescent proteins (FPs) have become indispensable
to biological
research. Since the discovery of the first FP, Aequorea
victoria green fluorescent protein (avGFP), new fluorescent
proteins have been continuously being developed. To achieve optimal
experimental results, selecting an FP based on specific characteristicssuch
as brightness, photostability, photochromicity, monomericity, pH robustness,
and fluorescence lifetimeis essential. However, a thorough
side-by-side comparison of these characteristics is missing for the
latest generation of FPs. Here, we provide a comprehensive characterization
in yeast of the most recently developed FPs, including FPs that were
codon-optimized for yeast. We believe that this provides an excellent
compendium for choosing the most suitable FP for research purposes.