Species identified by quantitative culture.
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Agar media used: MAC (MacConkey agar) for selection of enteric and bile-resistant non-fermenter species; MAC+TET (MacConkey agar with 10 µg/mL of Tetracycline) to select for Proteus vulgaris. CAN (Colistin Naladixic Acid agar with 5% sheep's blood on a Columbia agar base) for suppression of enteric species; BKV (Brucella-Kanamycin-Vancomycin agar) for selection of aminoglycoside+vancomycin-resistant Lactobacilli;1Aerobic incubation conditions were in 5% CO2 humidified atmosphere at 37°C; plates read at 24 and 48 hours of incubation.2Anaerobic conditions were in a Coy anaerobic chamber at 37°C. Plates were read in the chamber at 72 hours of incubation.
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2015-12-02



