RNA-seq expression profiling of Suz12-deleted or wild-type organoids derived from basal or luminal mouse mammary epithelial cells.

Total RNA was extracted from organoids grown from single sorted luminal or basal populations from the mammary glands of Rosa26-creERT2 Suz12 flox female mice. Cultures were treated with 4-hydroxytamoxifen to induce deletion of Suz12. Overall design: Two biological replicates were prepared of the basal-derived organoids and three biological replicates of luminal-derived organoids. Wild-type and Suz12-deleted samples were prepared from both basal and luminal organoids. RNA sequencing was carried out on an Illumina Nextseq 500 to produce 20-65 million 80 bp reads per sample.