We used single cell RNA sequencing to analyze the diversity of T cells in PBMC Overall design: T cells from healthy donor and patient were isolated by FACS using scRNA-seq
TN: naïve T cells (CD45RO-/CD62L+); TEF: effector T cells (CD45RO-/CD62L-); TCM: central memory T cells (CD45RO+/CD62L+high); TEM: effector memory T cells (CD45RO+/CD62L-); UNS: unstimulated cultures;