Microbial community analysis of supragingival plaque in patients with fixed prostheses

Background: Harmony between prostheses and periodontal tissues is essential, and in recent years, there has been much evidence that the presence of fixed prostheses increases the risk of periodontal lesion onset in abutment teeth and affects the longevity of fixed prostheses.Results:We collected 60 supragingival plaque samples from 40 people. The V3-V4 high variant region of the 16S rDNA gene was sequenced using high-throughput sequencing technology. The microbial composition and structural diversity of supragingival plaque on the surface of fixed prostheses were found to differ from that of normal natural crowns, with relatively high levels of periodontally related pathogens such as g Treponema, g Selenomonas, g Porphyromonas, and g Prevotella 7. Functional analysis revealed that the fixed prosthesis group had relatively active microbial communities. Metabolism was relatively active, with a high percentage of Lipopolysaccharide Biosynthesis, Clycine, Serine, and Treonine metabolism associated with the development of periodontal disease. The microflora on the surface of all-ceramic crowns was more similar to that of natural crowns than to that of porcelain-fused-mental crowns.Conclusion:Understanding the composition and differences between supragingival plaque biofilm microbes on the surface of fixed prostheses and natural crowns can provide patients with targeted guidance to focus on oral hygiene habits, reduce the risk of periodontal disease, improve the success rate of fixed prostheses, and prolong the service life of restorations.