scRNAseq of activated cells of the B cell lineage in the tumor microenvironment

The goals of this study were to gain a better understanding of the heterogeneity and transcriptional profile of activated cells of the B cell lineage in the tumor microenvironment. Overall design: Activated cells of the B cell lineage (live CD3/14/16-CD19+IgD-CD71+) from primary tumors and metastatic lymph nodes of 3 HPV+ HNSCC patients and, as comparison, from PBMC of a healthy individual 7 days post seasonal Influenza vaccination were FACS-purified. Single-cell suspensions of FACS-purified cells were loaded onto the 10X Genomics Chromium Controller. Library construction was performed using the Chromium Single Cell 5' Library Construction Kit followed by sequencing on a HiSeq3000 or NovaSeq6000.