A 31-Parameter Panel for Comprehensive Immunophenotyping of Multiple Immune Cells in Human PBMCs

The purpose of this work is to design a 29-color flow cytometry panel to capture the effects of aging on immune signature in human peripheral blood mononuclear cells (PBMCs).
Conclusion:
The panel designed in our study was an effective tool for analyzing age-related changes in each cell subset. We hypothesized that by comprehensively measuring immune cells, it would be possible to evaluate the interactions between each cell population and the differences in the timing of age-related changes. Our findings supported our hypothesis by demonstrating that it was possible to capture the immune status of individuals and to use high-dimensional analysis not only to understand specific cell populations but also to observe the whole picture of immune dynamics. All antibodies were titrated to optimize the concentration of antibodies. Quality control of the BD FACSymphony flow cytometer was regularly performed using BD CS&T beads.