Seasonal Transcriptome Profile Across Three Different Tissues of the Andean Killifish Orestias ascotanensis

These data support the unpublished manuscript <i>"Seasonal Transcriptome Profile Across Three Different Tissues of the Andean Killifish Orestias ascotanensis"</i> (Maldonado et al., Manuscript ID: SDATA-24-04380).<b>1. Genome Assembly and Annotation</b><b>Genome Assembly of Orestias ascotanensis.fa</b>: Reference genome in FASTA format (originally published in Di Genova et al. 2022).<b>annotation_Orestias (1).gtf</b>: Structural annotation (GTF format) of gene models, used for RNA-seq alignment and transcript quantification.<b>03_Gene_Annotation_Orestias_ascotanensis.csv</b>: Detailed functional annotation of protein-coding genes (PCGs), including:Genomic coordinates (scaffold, start, end, strand).Gene/transcript IDs, assigned names, and protein products.Functional terms: InterPro domains, Gene Ontology (GO), KEGG Orthology (KO), and EC numbers.<b>2. Transcriptomic Data</b><b>01_sample_info.csv</b>: Metadata for RNA-seq samples (tissue, season, replicates, etc.).<b>02_PCG_counts.csv</b>: Raw read counts for all protein-coding genes across samples.<b>04_DEGs.xlsx</b>: Differentially expressed genes (DEGs) across conditions, with fold-change, p-values, and adjusted p-values.<b>Table1_MappingStatistics.xlsx</b>: Sequencing and mapping statistics, including read quality metrics, alignment rates, and sample-specific processing details.<b>3. Long Non-Coding RNA (lncRNA) Predictions</b><b>Predicted_lncRNAs_Genomic_Locations_Orestias_ascotanensis.zip</b>: BED files with genomic coordinates of lncRNAs predicted by four tools (CPAT, TransDecoder, FEELnc, RNAmining), including scaffold, coordinates, strand, and transcript IDs (where available).