Genome-wide chromatin binding of YBX1 in nasopharyngeal carcinoma (NPC) cell line S18 (ChIP-Seq)

The development of therapeutic resistance and metastatic dissemination takes place in 20-30% patients with nasopharyngeal carcinoma (NPC), resulting in poor survival. Hence, a better understanding of the underlying molecular mechanisms will help improve clinical outcome. We have identified a novel circRNA, circIPO7, as a promoter of metastasis and cisplatin chemoresistance in NPC cells. circIPO7 was found to bind to YBX1. Genome binding/occupancy profiling by high throughput sequencing were performed to explore the underlying molecular mechanisms. Chromatin Immunoprecipitation (ChIP) was performed in the human nasopharyngeal carcinoma cell line S18 using YBX1 antibody, and then subjected to ChIP sequencing.