Diffusion coefficients (D) of labeled free oligonucleotides (probe), YOYO-1 iodide509-labeled in vitro-transcribed RNA, and DyLight 488-labeled virus.

The mean values and the standard deviation were calculated from at least 5 separate experiments with at least 20 repetitions each.1Mean of 3′ and 5′ probes.Diffusion coefficients calculated assuming different shapes:+sphere;*rod;†random coil. Note that virus with partially extruded RNA was not available in pure form and thus its diffusion constant could not be measured. The value of native virus was used instead in all experiments.