Urinary exosomal miRNA-663a shows variable expression in diabetic kidney disease patients with or without proteinuria

HK-2 cells were cultured in low and high glucose conditions for 96 hours in serum free conditions. Exosomes were isolated from the supernatant of these cells. miRCURY LNATM Universal RT microRNA PCR Human panel I+II (Exiqon) were used to study the differential expression from three different high glucose treated exosomes (HE1, HE2, HE3) than to low glucose treated exosomes (LE1, LE2, LE3). qPCR miRNA expression profiling from three different exosomal samples. Each sample was isolated from low and high glucose treated HK-2 cells