Pairwise Rousset’s distance between male (y-axis) and female (x-axis) tortoises in the Bolson tortoise captive breeding population.

New individuals that have been confirmed as pure G. flavomarginatus and that may be incorporated in to the breeding population are italicized. Negative values are effectively zero (no genetic differentiation); positive values (bold) indicate greater genetic differentiation between individuals. Individuals with low to no genetic differentiation could potentially be siblings or parent/offspring. Data generated using program SPAGeDi (Version 1.4) [35].