Results of combined predictions in discrimination of G+1-dependent 3′ss from G+1-independent 3′ss.

aEach combined prediction was considered positive if two or more of the three predicted values exceeded the herein proposed cut-off values of the individual tools. bThe sensitivity and specificity was calculated using Fu-mut set of G+1 mutations, containing artificially mutated PPTs. cThe sensitivity and specificity calculated using combined sets: test set and borderline set of G+1 mutations. Py25 = number of pyrimidines in the 25 nucleotides upstream from splice site; ME s.d. = difference between wild type and mutant sequence scores predicted by MaxEnt program; ME p.d. = difference between wild type and mutant sequence percentiles predicted by MaxEnt program; PSSM s.d.: accordingly.