BIO407 Group2 Image processing of live cells, treated with DFX

## Study:This data was acquired for the paper:Gottwald EM, Schuh CD, Drücker P, Haenni D, Pearson A, Ghazi S, Bugarski M, Polesel M, Duss M, Landau EM, Kaech A, Ziegler U, Lundby AKM, Lundby C, Dittrich PS, Hall AM. The iron chelator Deferasirox causes severe mitochondrial swelling without depolarization due to a specific effect on inner membrane permeability. Sci Rep. 2020 Jan 31;10(1):1577. doi: 10.1038/s41598-020-58386-9. PMID: 32005861; PMCID: PMC6994599.

## Study Component:Part of Fig.1c&dConfocal timelapse of Deferasirox treated cells, showing mitochondria swelling over time and polarization loss.

## Biosample:Opossum kidney (OK) cells (kind gift from the group of Prof O. Devuyst (Physiology, University of Zurich))

## Specimen:DFX treatment: 200uMDyes:-Mitochondria-GFP BacMam 2.0-TMRM (mitochondrial membrane potential dependent dye)

## Image Acquisition:Images were acquired using a Leica SP8 inverse STED 3x.Acquired channels:Channel 1: Mitochondria-GFP, 488nm excitation, 493nm-548nm emissionChannel 2: TMRM, 553nm excitation, 564nm-650nm emissionObjective: HC PL APO CS2 100x/1.40 OIL

## Image Data:151106_slide1_bacmam20_PPC_7473_Esther_DH_Progression.lif

Original project number:7473