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Data from: Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail

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Mendeley Data2024-06-25 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.cv0js0f
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资源简介:
Exp1_eggIt contains the data of egg laying in Exp. 1. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or iso-paired). Treatment2: assigned treatment for each replicate (isolated or paired). FirstEgg: the week eggs are found in the container of each replicate. DurationEgg: the number of weeks from FirstEgg until the end of experiment. TotalNegg: the total number of egg masses found in the container. RelEgg: the relative number of egg mass produced by one individual in a week. Week4-10: the number of egg masses found in the container in each week. Remarks: remarks for some replicates. We excluded several replicates for analyses, due to the absence of egg laying or low background mortality.Exp1_SLIt contains the data of body size in Exp. 1. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or iso-paired). Treatment2: assigned treatment for each replicate (isolated or paired). Shell length (mm): shell length of each snail at the end of experiment.Exp2_eggIt contains the data of egg laying in Exp. 2. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or grouped). #ind: the number of individuals in a container. Batch: identity of batch. FirstEgg: the day that first eggs are found in the container of each replicate. DurationEgg: the number of days from the FirstEgg until the end of experiment. TotalNegg: the total number of egg masses found in the container. Total/Treat: the number of egg masses laid by each individual. RelEgg: the relative number of egg mass produced by one individual in a day. Day25-62: the number of egg masses found in the container in every four days. Remarks: remarks for some replicates. We excluded several replicates for analyses, due to the absence of egg laying or low background mortality.Exp2_SLIt contains the data of body size in Exp. 2. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or grouped). Week: the week their body size was measured. Shell length (mm): shell length of each snail.Exp1_raw.geneIt contains the raw data of qPCR in Exp. 1. Plate: qPCR run identity. Running date: the date of each qPCR run. Well: well identify. Target: the identify of gene (SFP genes and house-keeping genes). Content: the assigned content of each well [Unknown, Negative Control (Neg Ctrl), Plate control (Plate ctrl)]. r.ID: randomly assigned sample identity to mask the true identity of each sample during qPCR. For the plate control, it also shows the dilution factor. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or iso-paired). Ct: the output of qPCR.Exp1_gene.rctIt contains the data of relative expression for SFP genes in Exp. 1. r.ID: randomly assigned sample identity to mask the true identity of each sample during qPCR. Treatment: assigned treatment for each replicate (isolated, paired or iso-paired). log(2dct_Lhis): relative expression of SFP genes, normalized by a house-keeping gene (Lhis).Exp2_raw.geneIt contains the raw data of qPCR in Exp. 2. Plate: qPCR run identity. Running date: the date of each qPCR run. Well: well identify. Target: the identify of gene (SFP genes and house-keeping genes). Content: the assigned content of each well [Unknown, Negative Control (Neg Ctrl), Plate control (Plate ctrl)]. r.ID: randomly assigned sample identity to mask the true identity of each sample during qPCR. For the plate control, it also shows the dilution factor. ID: identify of each replicate. Treatment: assigned treatment for each replicate (isolated, paired or grouped). Ct: the output of qPCR.Exp2_gene.rctIt contains the data of relative expression for SFP genes in Exp. 1. r.ID: randomly assigned sample identity to mask the true identity of each sample during qPCR. Treatment: assigned treatment for each replicate (isolated, paired or grouped). log(2dct_mean.ref): relative expression of SFP genes, normalized by all three house-keeping gene.
创建时间:
2023-06-28
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