RT polymorphic substitutions (Sanger) compared to reference HIV-1 B in isolates from naïve patients and amounts of K65R and K70R mutations in the same isolates plus control plasmids.

Footnote to Table 2. Two subtype C MJ4 plasmids, one with K65K (wild type) and one with K65R were used to amplify RT region before sequencing by UDPS. Amplicon pools were not prepared in equimolar concentrations but with different percentages of K65R mutation. The theoretical and observed values of K65R plasmid dilutions were 100%∶94%, 5%∶2.30% and 1%∶0.90%.