MolMet Supp 2: Gckr transcript-1 is the predominant transcript expressed in mouse liver and mouse primary hepatocytes.

A-C) Relative abundance of Gckr transcripts-1,2, X1 and X2 in mouse tissues determined using primer sets targeting either a common sequence (spanning exons 14-15) or spanning the deletion sites of transcript-2, X1 and X2.
A) Relative selectivity of the 4 primer sets determined against RNA extracts from cells transfected with adenoviral vectors (10x106 pfu/ml) encoding the 4 transcripts: Gckr-t1 (RefSeq XM_006503881.3), Gckr-t2 (RefSeq BC012412), Gckr-X1 (RefSeq XM_006503882) and Gckr-X2 (XM_006503883).
B) Gck and Gckr mRNA are expressed at highest levels in liver.
C) Gckr-transcript-1 is the predominant transcript in liver and hepatocytes.
(B-C) Means ± SEM for n=4-8 extractions.
D) Amino acid alignment of mouse GKRP isoforms 1 and 2, predicted isoforms X1 and X2 versus human and rat sequences using Clustal Omega and visualized via Esprit3(Robert, X. and Gouet, P. (2014) "Deciphering key features in protein structures with the new ENDscript server". Nucl. Acids Res. 42(W1), W320-W324 - doi: 10.1093/nar/gku316). Showing deleted regions of Isoform 2, X1, and X2.

A-C) 通过针对共同序列（跨越外显子14-15）或跨越转录本-2、X1和X2的缺失位点的引物集，在鼠标组织中确定Gckr转录本-1、2、X1和X2的相对丰度。A) 通过针对编码4种转录本（Gckr-t1（RefSeq XM_006503881.3）、Gckr-t2（RefSeq BC012412）、Gckr-X1（RefSeq XM_006503882）和Gckr-X2（XM_006503883）的腺病毒载体（10x10^6 pfu/ml）转染的细胞RNA提取物，确定4种引物集的相对选择性。B) Gck和Gckr mRNA在肝脏中表达水平最高。C) Gckr转录本-1是肝脏和肝细胞中的主要转录本。（B-C）表示n=4-8次提取的平均值±标准误。D) 使用Clustal Omega进行氨基酸比对，将小鼠GKRP同种异构体1和2、预测的同种异构体X1和X2与人类和鼠序列进行比对，并通过Esprit3（Robert, X. 和 Gouet, P. （2014）“利用新的ENDscript服务器解析蛋白质结构中的关键特征”。Nucleic Acids Res. 42(W1)，W320-W324 - doi: 10.1093/nar/gku316）进行可视化。显示同种异构体2、X1和X2的缺失区域。