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RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in IPEX and healthy donors isolated from peripheral blood.

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https://www.ncbi.nlm.nih.gov/sra/SRP306714
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RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in IPEX and healthy donors isolated from peripheral blood. Overall design: Human Tregs and Tconvs from IPEX and healthy donors were sorted from PBMCs as DAPI– CD4+CD25hiCD127lo and CD4+CD25–CD127hi, respectively. 1,000 cells were double-sorted directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org). Samples were collected and sequenced in two cohorts.
创建时间:
2021-05-26
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