M. agalactiae CDSs identified by high-throughput screening for their reduced growth capacities on cultured cells.

aCDS found disrupted in M. agalactiae growth-deficient mutants [6].bLetters E and F indicate that the M. agalactiae growth-deficient mutants were selected on TIGMEC or TIGEF cells, respectively. Asterisks (*) indicate mutants that were also selected during high-throughput screening on HeLa cells.cFor each CDS, the number of mutants identified during the screening on caprine cell lines is indicated, as well as the number of different mini-Tn insertion sites.dFor each CDS, the relative position and the orientation of the inserted transposon are indicated. Mini-Tn insertion sites were determined by direct sequencing of genomic DNA, and their positions were defined based on the published genome sequence (NC_009497).eHypothetical proteins (HP) have no homolog outside the species M. agalactiae. Conserved hypothetical proteins (CHP) share sequence similarity with proteins of unknown function identified in other Mollicutes or other bacteria. COG categories of encoded proteins are indicated in parentheses [38].fProtein localization was predicted using TMHMM [41], [42]; membrane (M), cytosolic (C), or indirectly linked to the membrane (IM).gProteins with peptides detected during proteomic analysis of gene products expressed by M. agalactiae strain PG2 in axenic culture are identified by a plus sign (+), while proteins not detected are identified by a minus sign (−) [32].