Single cell gene expression from the hippocampus of 5xFAD mice subjected to stroke

Neurogenesis after stroke compounded with amyloid beta plaques around the cerebral vasculature has implications in delaying tissue recovery. We used single cell RNA sequencing (scRNA-seq) to understand the cellular interactions in the hippocampus during post-stroke recovery with CAA. Overall design: The hippocampus of 5xFAD or WT mice was isolated 7 days post-stroke. The contralateral and ipsilateral side were collected on ice in Hibernate A medium and homogenized into a single cell suspension for analysis using scRNA-seq.