Determination of WatR binding site in Methylomonas sp. DH-1 after lactate adaptive evolution. Determination of WatR binding site in Methylomonas sp. DH-1 after lactate adaptive evolution

JHM80 strain was obtained by adaptive evolution of Methylomonas sp. DH-1 strain against 8.0 g/L of lactate. The tolerance against lactate of JHM80 was obtained by the overexpression of watR (weak acid tolerance regulator). In order to discover the target genes of WatR, FLAG epitope tag was added to WatR of JHM80 strain and ChIP-seq was performed. Overall design: In order to discover the target genes and binding consensus of WatR, ChIP-seq was performed in the normal growth condition.