Expression data from Arabidopsis thaliana Col-0 transformed with constructs misexpressing NAC052 transcription factor involving leaf morphology and anatomy

In an effort to identify genetic regulators for the cell ontogeny around the veins in Arabidopsis thaliana leaves, an activation-tagged mutant line with altered leaf morphology and altered bundle sheath anatomy was characterized. This mutant had a bundle sheath-specific promoter from the gene GLYCINE DECARBOXYLASE SUBUNIT-T from the C4 species Flaveria trinervia (GLDTFt promoter) inserted in the coding region of the transcriptional repressor NAC052. Reconstruction of the mutation event of our activation-tagged line by creating a line expressing an amino-terminally truncated sequence of NAC052 under control of the GLDTFt promoter as well as creating a line expressing the full sequence of NAC052 under control of the GLDTFT promoter, as well as creating a CRISPR/Cas mutant line of NAC052, confirmed the involvement of NAC052 in leaf development. Complete Arabidopsis rosettes of 5 lines (reference line, activation tagged line, GLDTFT::5'truncatedNAC052 line, GLDTFT::NAC052 line, and CRISPR/Cas nac052) were harvested on day 28 after sowing.