WGBS related files

DNA methylation, as well as histone modifications, is an important regulatory mechanism for altered gene expressions. Our previous study hasshown that Bombyx moricytoplasmic polyhedrosis virus (BmCPV)infection could change the level of trimethylation of lysine 9 of histone 3 (H3K9me3)and acetylation of lysine 9 of histone 3 (H3K9ac), thus regulating the mRNAs expressions in the midgut of silkworm, B.mori. However, the correlation between genome-scale DNA methylome and transcriptome remains underexplored. In this study, whole genome bisulfite sequencing (WGBS) was performed on the midgut of BmCPV-infected silkworms at 48 h and 96 h post infection, and corresponding midguts of uninfected silkworms. And intersection genes were screened by correlation analysis between differentially methylated regions (DMR)-associated genes and differentially expressed genes (DEGs). Above analysis will contribute to further understanding how BmCPV regulate gene expression through epigenetic modification at the genome-wide level.
In this item, DMR bed files and methylation calling related files are uploaded.