Glycine max Raw sequence reads

leaf is the main organ responsible for photosynthesis and photoperception, and plays an important role in plant growth and crop yield. Here, we identified a rolling-leaflet mutant (rl) of soybean. Compared with wild type HX3, rl showed rolling and narrow leaflet, and also revealed narrow pod and narrow seed. Anatomical and cytological analysis demonstrated that cell area of upper epidemis and leaflet thickness enlarged in rl, which may lead rolling and narrow leaf. Transcriptome analysis revealed that several cytokinin oxidase/dehydrogenase genes were up-regulation dramatically, which may cause lower cytokinin level in rl. And hormone treatment essay indicated that 6-BA rescued rolling leaf enough, and rescued narrow leaf partly. Our results suggest abnormal cytokinin metabolism caused rolling and narrow leaf in rl, and provide valuable clues for further understanding the mechanisms underlying leaf development in soybean.