Control of odor sensation by light and cryptochrome in the Drosophila antenna

Olfaction is a sense employed by insects to differentiate safe from harmful food options, evaluate potential mates, and identify oviposition sites. Here, we found that the fruit fly, Drosophila melanogaster, responds differently to a set of repulsive odors depending on ambient light conditions. Ultraviolet (UV) or blue light reduces the behavioral aversion and electrophysiological responses of olfactory receptor neurons (ORNs) to certain repellent odors, such as benzaldehyde. We found that cryptochrome (cry) is strongly expressed in the antennal support cells that lie adjacent to ORNs, and mutation of cry eliminates the light-dependent reduction in aversion. Thus, these data indicate that support cells in an olfactory organ serve a sensory function as light responsive cells. It has been shown that light activation of Cry creates reactive oxygen species (ROS), and ROS activates the TRPA1 channel. We demonstrate that the TRPA1-C isoform is expressed and required in ORNs for benzaldehyde r..., , , # Control of odor sensation by light and cryptochrome in the Drosophila antenna

Dataset DOI: [10.5061/dryad.ffbg79d5n](10.5061/dryad.ffbg79d5n)

## Description of the data and file structure

We tested the effects of ambient light on the olfactory behavior of Drosophila melanogaster. The following are video recordings, electrophysiology, immunohistochemistry and functional imaging that were performed to assay the olfactory behavior and physiology of Drosophila.

### Files and variables

#### File: Odorant-cap.stl

**Description:** 3D print file that prints the odorant-housing and assay-tube-capping implement used in the behavior assays in this work. This part is best printed using a resin-based printer on a fine precision setting.

#### File: Imaging-Chamber.stl

**Description:** 3D print file that prints the imaging chamber constructed to place one to two Drosophila antennae in place for functional imaging. This part is best printed using a resin-based printer in order to obtain smoot...,