Coordinated Action of Multiple Transporters in the Acquisition of Essential Cationic Amino Acids by the Intracellular Parasite Toxoplasma gondii (GC-MS Data set)

Intracellular parasites of the phylum Apicomplexa are dependent on the scavenging of essential amino acids from their hosts. We previously identified a large family of apicomplexan-specific plasma membrane-localized amino acid transporters, the ApiATs, and showed that the Toxoplasma gondii transporter TgApiAT1 functions in the selective uptake of arginine. TgApiAT1 is essential for parasite virulence, but dispensable for parasite growth in medium containing high concentrations of arginine, indicating the presence of at least one other arginine transporter. Here we identify TgApiAT6-1 as the second arginine transporter. Using a combination of parasite assays and heterologous characterisation of TgApiAT6-1 in Xenopus laevis oocytes, we demonstrate that TgApiAT6-1 is a general cationic amino acid transporter that mediates both the high-affinity uptake of lysine and the low-affinity uptake of arginine. TgApiAT6-1 is the primary lysine transporter in the disease-causing tachyzoite stage of T. gondii and is essential for parasite proliferation.

Here we provide an GC-MS data set of T.gondii extracellular parasites. Parasites cultured for two days in the absence or presence of the TgApiAT6-1 transporter in amino acid-free RPMI 1640 medium supplemented with [13C]-labelled amino acid mix. Polar metabolites were extracted and analysed by GC-MS. We compared the fractional abundance of [13C]-labelled amino acids to the total abundance of each amino acid following the 15 min uptake period. Of the 17 amino acids detected by GC-MS, only the uptake of [13C]-Lys was significantly reduced when TgApiAT6-1 expression was knocked down. This data set provides the raw and processed data files which accompanies Fig. 2A in the publication:

Coordinated Action of Multiple Transporters in the Acquisition of Essential Cationic Amino Acids by the Intracellular Parasite Toxoplasma gondii
Stephen J. Fairweather, Esther Rajendran, Martin Blume, Kiran Javed, Birte Steinhöfel, Malcolm J. McConville, Kiaran Kirk, Stefan Bröer, Giel G. van Dooren
bioRxiv 2021.06.25.450001; doi: https://doi.org/10.1101/2021.06.25.450001

This GC-MS data set contains:
1. Sample Summary Table (Excel file) of including samples IDs (numbered) corresponding to the uploaded raw data files.
2. A compressed (.zip) folder containing raw data folders for each sample numbered with the same sample ID as in the summary spreadsheet. Each folder contains the .D format raw and processed results files.