Primer pairs used for mutagenesis of CP-encoding sequence of PVYN or PVYO.
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aRestriction sites are in italic and underlined. The nucleotides introduced before the restriction sites increase the efficiency of digestion. Mutated nucleotides are in bold type. “P” indicates that the 5′-terminus of the primer is phosphorylated. Tm indicates melting temperature in Celsius degrees.Primer pairs used for mutagenesis of CP-encoding sequence of PVYN or PVYO.
创建时间：
2015-12-02
