Additional file 1 of Characterization of porcine sapelovirus prevalent in western Jiangxi, China

Additional file 1: Supplementary Fig. 1. Development of a SYBR Green I-based real-time PCR method for PSV detection. (a) Standard curves generated from the mean cycle threshold (CT) values obtained against the diluted plasmid standards (log 10 copy number). The correlation coefficient (R2) and the equation of the regression curve (Y) were calculated. Equation: y=-5.1538x+50.716; correlation coefficient: R2=0.9996. (b) Melting curve analysis of real-time PCR based on SYBR Green I. The Tm of PSV real-time PCR was 84.82 °C. (c) Specificity of the PSV SYBR Green I real-time PCR. Only the PSV-HuN1 strain showed a high-intensity fluorescent signal. CSFV, PRRSV, PRV, PEDV, PTV, and JEV did not show specific amplification. (d) Sensitivity of the SYBR Green I real-time PCR. The 10-fold serial dilutions of pMD19-T-PSV plasmids ranging from 5.22×108-5.22×101 copies/μL marked as 1-8, respectively. (e) The agarose gel electrophoresis results of conventional PCR. DNA marker of 5000 bp was used. The 10-fold serial dilutions of pMD19-T-PSV plasmids ranging from 5.22×108-5.22 copies/μL in lanes 1-9, respectively; lane 10 contains the negative control.