HTS data from in vivo prime editing

NIAID Data Ecosystem2026-03-14 收录

基因编辑验证

AAV腺相关病毒

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA898625 数据链接链接失效反馈

官方服务：

资源简介：

Efficient AAV-mediated in vivo prime editing

创建时间：

2022-11-05

POLR2J4 large deletion confirmed at the single clone level.

基因编辑验证

单克隆细胞系分析

(A) Gel electrophoresis of NGS-derived PCR amplicons shows large deletions of POLR2J4 in clones #6 and #7 samples. (B) POLR2J4 indel rate. M indicates the normal cell line. #6 indicates a cell line wi

Figshare2025-05-16 更新80

ONT long read sequencing to validate prime editing efficiency and off targets

基因编辑验证

高通量测序

ONT long read sequencing to validate prime editing efficiency on target, PAM introcution and off targets. ONT_ID file links ONT IDs with the plasmid used in the study

Figshare2023-04-05 更新30

Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events - supplementary tables

基因编辑验证

脱靶效应检测

Supplementary tables

Figshare2023-05-10 更新20

Additional file 2: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

基因编辑验证

模型构建

Table S2. The results of the two-donor floxing approach via microinjection for 49 unique loci. (XLSX 21 kb)

Figshare2019-08-26 更新40

Statistical significance for NIL and CRISPR assays.