List of major antibodies and cocktail configurations used for MHC staining of rat, mouse, and human skeletal muscle.

All antibody cocktails are prepared in block solution (10% goat serum in PBS).*Non-specific cross-reactivity with MHCIIx was observed in human skeletal muscle.1Fibers positive for MHCIIx showed red staining, but for presentation and discussion purposes in rats and mice these fibers were pseudo-colored purple to avoid confusion with MHCIIb positive fibers.