miRNA expression profiles of uncultured stromal cells of eutopic and ectopic originÂ

We utilized fluorescence-activated cell sorting to isolate endometrial stromal cells from paired endometrial and endometrioma biopsies and combined it with high-throughput sequencing to determine miRNA alterations in endometriotic stroma. The analysis revealed 149 abnormally expressed miRNAs in endometriotic lesions, including extensive upregulation of miR-139-5p and downregulation of miR-375 compared to eutopic cells. The results of this study provide further insights into the complex molecular mechanisms involved in endometriosis pathogenesis and demonstrate the necessity for cell-type specific analysis of ectopic tissues to understand the interactions between different cell populations in disease onset and progression. Overall design: miRNA profiles of paired eutopic and ectopic endometrial stromal cells were generated by small-RNA deep sequencing using Illumina NextSeq 500 platform.