Data and code: Temporal stability of Synechococcus communities under thermal fluctuations

This deposit contains the raw data and analysis code underlying the manuscript. We tested how the acclimation responses of marine picocyanobacteria govern the temporal stability of the communities they form under a recurring warming–cooling regime. Twelve strains of Synechococcus sp. (Roscoff Culture Collection) were used to assemble 40 two- and three-strain microcosm communities spanning a broad range of two continuous community-level predictors: mean acclimation response and response diversity (mean pairwise Euclidean distance among constituent strains, sensu Ross et al. 2023). Both predictors were computed twice, from acute (CT − TC) and from chronic (TT − CC) strain-level responses. The experiment ran in three sequential phases: The experiment ran in three sequential phases: Phase Description Duration Purpose 1 Monoculture acclimation at control (C, 19 °C) or warming (T, 24 °C) 10 d Generate distinct acclimation histories 2 2 × 2 cross-exposure (CC, CT, TC, TT) 48 h Quantify acute and chronic strain responses 3 Thermal fluctuation regime, C₁→T₁→C₂→T₂ in 4-day blocks 16 d Measure community temporal stability Cell density and a per-cell chlorophyll a proxy (RED-B.HLin / FSC.HLin) were quantified by flow cytometry (Guava EasyCyte HT-7). Temporal stability is reported as 1/CV across the 16-day regime.