TEM detection and analysis of lipid bodies (LBs) in live L. am amastigote-hosting C57BL/6 mouse DLs.

Panel A: TEM pictures of DL cultures exposed or not to live L. am amastigotes and incubated or not with oleate. Representative pictures are shown for control (Ctrl; upper left panel), oleate-treated (Oleate, lower left panel), amastigote-loaded (L. am, upper right panel) and amastigote-loaded treated by oleate (L. am+ oleate, lower right panel) C57BL/6 DL cultures. LBs are indicated. Panel B: Analysis of LBs in control and live L. am amastigote-hosting C57BL/6 DLs incubated or not with oleate. LB were counted and their area determined in TEM section pictures. The results are represented as box and whisker plots. B1: number of cytosolic LBs in Ctrl DLs and DLs hosting live L. am amastigotes in the absence or presence of oleate. B2: LB area in Ctrl DLs and LDs hosting live L. am amastigotes in the absence or presence of oleate. Statistical analyses were performed by the Mann Whitney test after analysing at least 60 sections of DL samples. (*): p