After sampling for this study was completed in October 2016, the collaborating commercial beekeeper noticed increased deaths (over 60%) in the apiary where monitored colonies were located.

Therefore, additional samples were obtained (i.e., 10 samples from 9 colonies; both adult bees and brood were obtained from one colony) from weak or dead colonies on November, 2016. Only two of the colonies sampled were established monitor colonies (i.e., c5 and c24), and therefore this data was not analyzed with the sample cohort. In addition to the panel of 13 pathogens monitored in the original study, additional molecular tests for two bacterial pathogens Melissococcus plutonis and Paenibacillus larvae, the causative agents of European Foulbrood Disease and American Foulbrood Disease, respectively; and a fungus that cause Chalkbrood disease Ascosphaera apis were carried out on these samples. (XLSX)